Phospho-rps6
WebThe phosphorylation of ribosomal protein S6 (rpS6) has been described for the first time about four decades ago. Since then, numerous studies have shown that this modification …
Phospho-rps6
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WebMar 22, 2024 · Both phosphorylation sites of rpS6 (S235/236 and S240/244) were detected with phospho-specific antibodies. Total protein levels of rpS6, FLAG and β-actin were also … WebDec 21, 2024 · Ribosomal protein S6 (RPS6) is a component of the 40S small ribosomal subunit and participates in the control of mRNA translation. Additionally, phospho (p) …
WebFeb 6, 2024 · We developed single-cell RNA and Immunodetection (RAID) to allow combined analysis of the transcriptome and intracellular (phospho-)proteins from fixed single cells. RAID successfully... WebCells were then intracellularly stained with 5 µg/mL (1:100 dilution) of Purified anti-RPS6 Phospho (Ser244) Antibody overnight at 4°C, after which proteins were visualized with Alexa Fluor® 594 Goat anti-mouse IgG Antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
WebQuality Control: the Phospho-RPS6 (S240/S244) assay kit is routinely tested against Insulin-treated MCF7 lysates. MCF7 cells were cultured in a T175 flask to 90% confluence and stimulated with 10 µM of insulin for 60 min at RT. Following cell lysis using 16 mL of 1X Lysis Buffer 4, lysates WebRPS6 Rabbit Polyclonal Antibody: 50μl: 593.00元: 加入购物车: AG3129: Phospho-S6 Ribosomal Protein (Ser235/236) Mouse Monoclonal Antibody: 50μl: 998.00元: 加入购物车: AG3133: Phospho-S6 Ribosomal Protein (Ser235/Ser236) Rabbit Monoclonal Antibody: 50μl: 998.00元: 加入购物车: AG3136: Phospho-S6 Ribosomal Protein (Ser240/244 ...
WebMay 3, 2024 · (i) Ribosomal protein S6 (rpS6) is a downstream substrate of PI3K/akt/mTOR/ kinase and MAPK kinase pathways and its dephosphorylation is also triggered by DNA double strand breaks. Phospho-rpS6 is reliably measurable by flow cytometry and thus has the potential to function as a biomarker of responsiveness to several therapeutic agents.
WebNational Center for Biotechnology Information divyendu sharma new web seriesWebp-rpS6 is a robust post-treatment indicator of HER2 pathway-targeted therapy resistance; Resistance to Selumetinib (AZD6244) in colorectal cancer cell lines is mediated by … divyesh bhaiWebPhospho-S6 Ribosomal Protein (Ser235/236) Antibody detects endogenous levels of ribosomal protein S6 only when phosphorylated at serine 235 … divyendu sharma twitterWebFeb 6, 2024 · RPS6 is phosphorylated downstream of active EGF signaling 13 and is lost within the first 24 hours of AG1478 treatment (Supplementary Fig. 4A). Quantification of … craftsman v20 cordless impact wrenchWebAn immobilized capture antibody specific for Ribosomal Protein S6 / RPS6 binds both phosphorylated and unphosphorylated Ribosomal Protein S6 / RPS6. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format. Complete Your Research craftsman v20 cordless lawn mowerWebResults: The activity of a downstream effector of the HER2 pathway phosphorylated ribosomal protein S6 (p-rpS6), was suppressed by trastuzumab in the parental cell lines … divyesh ginoyaWebAnimals were fasted overnight and refed ad lib for 4 h. Quantification of phospho-rpS6 is shown at the right, normalized to total rpS6. (*) P < 0.05 by Student's t-test. (D) Immunofluorescence staining of phospho-S6 (red) and TFE3 (green) in control (NTC) cells and cells lacking TSC1 or TSC2. Cells were starved or starved and restimulated with ... divyesh ghelani